5-Amino-1MQ | Buy Research-Grade 5-Amino-1MQ in Europe | ≥99% Purity

5-Amino-1MQ (5-Amino-1-methylquinolinium) is a synthetic small molecule NNMT (Nicotinamide N-methyltransferase) inhibitor, available to buy in Europe for laboratory research into metabolic regulation, adipogenesis, NAD+ biology, epigenetic signalling, and the emerging field of NNMT inhibition as a pharmacological strategy for studying obesity-associated metabolic dysfunction.

Laboratories and research institutions across the EU can order verified, research-grade 5-Amino-1MQ with fast international dispatch to Europe, full batch documentation, and ≥99% purity confirmed by HPLC and Mass Spectrometry.

✅ ≥99% Purity — HPLC & Mass Spectrometry Verified

✅ Batch-Specific Certificate of Analysis (CoA)

✅ High-Purity Small Molecule | GMP Manufactured

✅ Fast Dispatch to EU & Europe | Tracked Shipping

What is 5-Amino-1MQ?

5-Amino-1MQ (5-Amino-1-methylquinolinium) is a synthetic small molecule inhibitor of Nicotinamide N-methyltransferase (NNMT) — an enzyme that catalyses the methylation of nicotinamide (a form of vitamin B3) using S-adenosylmethionine (SAM) as the methyl donor, producing 1-methylnicotinamide (1-MNA) and S-adenosylhomocysteine (SAH) as products. 5-Amino-1MQ is a charged quinolinium compound that competitively inhibits NNMT by occupying the nicotinamide binding site of the enzyme — blocking its catalytic activity with high potency and selectivity relative to other methyltransferases.

NNMT is an enzyme of significant and growing research interest in metabolic biology — expressed most highly in white adipose tissue and liver, but also found in skeletal muscle, kidney, and multiple cancer tissue types. Its metabolic significance stems from its position at the intersection of two central metabolic regulatory systems: the NAD+ salvage pathway and the SAM-dependent methylation cycle. By consuming nicotinamide — a NAD+ precursor — and SAM — the universal methyl donor — NNMT activity influences both cellular NAD+ availability and the methylation potential of the cell, with downstream consequences for epigenetic regulation, sirtuin activity, and the gene expression programmes controlling metabolic function.

In adipose tissue specifically, NNMT has been characterised as a metabolic regulator whose activity increases in obesity — with elevated adipose NNMT expression associated with reduced NAD+ levels, impaired sirtuin activity, and a gene expression profile favouring fat storage over oxidative metabolism. Studies have documented that NNMT inhibition in adipocytes and adipose tissue drives a shift toward reduced fat cell size, enhanced fatty acid oxidation, increased energy expenditure, and resistance to diet-induced obesity in pre-clinical models — establishing NNMT as a research target of significant interest in obesity and metabolic disease biology.

5-Amino-1MQ’s well-characterised NNMT inhibition profile, cell membrane permeability, and documented metabolic effects in pre-clinical models have made it the most widely used NNMT inhibitor research tool available to European laboratories — providing a practical small molecule probe for studying NNMT biology across metabolic, epigenetic, and cancer research applications.

What Does 5-Amino-1MQ Do in Research?

In laboratory settings, 5-Amino-1MQ is studied primarily as an NNMT inhibitor with downstream consequences for NAD+ metabolism, methylation biology, and adipose tissue function — with research applications extending into epigenetic regulation, cancer biology, and metabolic disease models. EU and European researchers working with 5-Amino-1MQ typically focus on:

• NNMT inhibition and enzyme pharmacology research — 5-Amino-1MQ is the reference small molecule NNMT inhibitor, used in enzyme kinetics studies, inhibition mechanism characterisation, selectivity profiling against related methyltransferases, and structure-activity relationship research examining how quinolinium modifications influence NNMT binding and inhibitory potency.
• NAD+ biology and salvage pathway research — NNMT consumes nicotinamide — a key NAD+ precursor in the salvage pathway — and its inhibition by 5-Amino-1MQ increases nicotinamide availability for NAD+ synthesis. Studies use 5-Amino-1MQ to examine how NNMT-mediated diversion of nicotinamide influences cellular NAD+ levels, NAD+/NADH ratios, and the downstream consequences for NAD+-dependent enzyme activity in metabolic research.
• Sirtuin activity and epigenetic research — NAD+ is an obligate co-substrate for sirtuin deacylases — the NAD+-dependent epigenetic regulators including SIRT1 that influence histone modification, gene expression, and metabolic adaptation. 5-Amino-1MQ’s effect on NAD+ availability through NNMT inhibition positions it as a research tool for studying how NNMT activity influences sirtuin function and the epigenetic programmes downstream of NAD+-dependent deacylation.
• Adipogenesis and adipocyte biology research — studies have characterised 5-Amino-1MQ effects on adipocyte differentiation, lipid accumulation, and the gene expression programmes governing fat cell biology — with findings documenting inhibition of adipogenesis and reduction of adipocyte lipid content in cell culture models. These findings have established 5-Amino-1MQ as a research tool for studying NNMT’s role in adipose tissue biology.
• Obesity and metabolic disease pre-clinical research — pre-clinical studies have examined 5-Amino-1MQ in dietary obesity models — documenting effects on body weight, adipose depot mass, metabolic rate, and related parameters in the context of NNMT inhibition-driven metabolic reprogramming. These findings have positioned 5-Amino-1MQ as a pharmacological research tool for studying the contribution of NNMT activity to obesity-associated metabolic dysfunction.
• SAM methylation potential and epigenetics research — NNMT consumes SAM — reducing the SAM/SAH ratio and the methylation potential of the cell. Studies use 5-Amino-1MQ to examine how NNMT-mediated SAM consumption influences the methylation reactions regulating histone methylation, DNA methylation, and the epigenetic landscape of metabolically relevant tissues.
• Energy expenditure and thermogenesis research — studies have characterised 5-Amino-1MQ effects on cellular energy expenditure parameters — including oxygen consumption rates, mitochondrial function markers, and thermogenic gene expression — in the context of NNMT inhibition-driven shifts in adipose tissue metabolic programme.
• Cancer biology research — NNMT is overexpressed in multiple cancer types — including glioblastoma, gastric cancer, oral cancer, and others — where elevated NNMT activity has been associated with tumour progression, invasion, and therapy resistance. 5-Amino-1MQ is used as a research tool for studying NNMT’s contribution to cancer cell biology and the effects of its inhibition on cancer cell metabolic and epigenetic programmes.
• Skeletal muscle metabolism research — NNMT expression in skeletal muscle has been studied in the context of insulin resistance and muscle metabolic dysfunction — with 5-Amino-1MQ used to probe NNMT’s contribution to muscle NAD+ biology and the metabolic consequences of its inhibition in muscle tissue models.
• Methylation cycle and one-carbon metabolism research — 5-Amino-1MQ’s effect on SAM consumption connects NNMT inhibition to the broader one-carbon metabolism network — making it a research tool for studying how NNMT activity interfaces with folate cycle biology, homocysteine metabolism, and the cellular methylation reactions central to epigenetic regulation.
• Comparative NNMT inhibitor pharmacology — 5-Amino-1MQ serves as the primary reference compound for NNMT inhibitor research — benchmarked against other NNMT inhibitors in development to characterise potency, selectivity, cell permeability, and metabolic effects in comparative enzyme inhibitor pharmacology studies.

All research applications are for in vitro and pre-clinical use only.

What Do Studies Say About 5-Amino-1MQ?

5-Amino-1MQ has generated a growing and high-quality research literature since its characterisation as an NNMT inhibitor — with pre-clinical studies establishing its pharmacological profile and documenting metabolic, epigenetic, and cancer biology effects that have positioned it as the leading small molecule tool for NNMT research.

NNMT inhibition characterisation: Foundational biochemical studies characterised 5-Amino-1MQ as a potent competitive NNMT inhibitor — documenting its binding to the nicotinamide substrate site of NNMT with nanomolar to low micromolar potency and establishing its selectivity profile relative to related methyltransferases. These studies established 5-Amino-1MQ as the reference NNMT inhibitor for pre-clinical research and provided the mechanistic basis for interpreting its downstream biological effects.

Adipose tissue and obesity research: A landmark pre-clinical study examined 5-Amino-1MQ in a high-fat diet obesity model — documenting significant reductions in body weight gain, adipose depot mass, and metabolic dysfunction parameters in treated animals compared to controls. The study characterised effects on adipocyte size, metabolic gene expression in adipose tissue, and systemic metabolic parameters — providing the foundational pre-clinical evidence for NNMT inhibition as a metabolic research strategy and establishing 5-Amino-1MQ as the primary tool for this research application.

NAD+ and sirtuin biology: Studies have documented 5-Amino-1MQ-mediated increases in cellular nicotinamide availability and NAD+ levels in treated cells and tissues — with downstream consequences for SIRT1 activity and the epigenetic programmes regulated by NAD+-dependent sirtuin deacylation. These findings have connected NNMT inhibition to the extensively studied NAD+/sirtuin axis in metabolic and ageing biology — positioning 5-Amino-1MQ as a complementary research tool to direct NAD+ precursor supplementation strategies.

Adipogenesis inhibition: Cell culture studies have documented 5-Amino-1MQ inhibition of adipocyte differentiation — with findings showing reduced lipid accumulation, altered adipogenic gene expression including downregulation of PPARγ and FABP4, and changes in the epigenetic landscape of differentiating pre-adipocytes consistent with NNMT inhibition-driven methylation potential changes. These findings have contributed to mechanistic understanding of how NNMT activity regulates the adipogenic programme.

Cancer biology research: Studies examining 5-Amino-1MQ in cancer cell models have characterised effects on proliferation, migration, and metabolic parameters in NNMT-overexpressing cancer cell lines — with findings suggesting that NNMT inhibition reduces the metabolic and epigenetic advantages conferred by elevated NNMT activity in cancer cells. These findings have contributed to growing research interest in NNMT as a cancer metabolism target.

Methylation potential effects: Studies have characterised 5-Amino-1MQ-mediated changes in the SAM/SAH ratio in treated cells — documenting increased methylation potential consistent with reduced NNMT-mediated SAM consumption — with downstream consequences for histone methylation marks and transcriptional programmes in metabolically relevant tissues. These epigenetic findings have connected NNMT inhibition to chromatin biology and provided mechanistic context for the gene expression changes observed in 5-Amino-1MQ-treated cells.

Muscle biology research: Studies examining 5-Amino-1MQ in skeletal muscle cell models have characterised effects on NAD+ metabolism, mitochondrial function parameters, and insulin signalling components — contributing to understanding of how NNMT activity in muscle tissue influences metabolic function and the consequences of its inhibition for muscle metabolic biology.

5-Amino-1MQ vs Related Metabolic and NAD+ Biology Research Compounds

Compound	Type	Target	Primary Research Application
5-Amino-1MQ	Small molecule NNMT inhibitor	NNMT enzyme	Adipose biology, NAD+ salvage, epigenetics, obesity research
NMN	NAD+ precursor	NAD+ salvage pathway	Direct NAD+ elevation, sirtuin biology, ageing research
NR (Nicotinamide Riboside)	NAD+ precursor	NAD+ salvage pathway	NAD+ biology, mitochondrial function, ageing
MOTS-c	Mitochondria-derived peptide	AMPK, folate cycle	Metabolic regulation, insulin sensitivity, exercise biology
SLU-PP-332	Pan-ERR agonist	ERRα/β/γ	Mitochondrial biogenesis, exercise mimetic biology
Resveratrol	SIRT1 activator	SIRT1 / PGC-1α	NAD+/sirtuin axis, mitochondrial biology

Buying 5-Amino-1MQ in Europe — What’s Included

Every order of 5-Amino-1MQ dispatched to EU and European research institutions includes:

• Batch-Specific Certificate of Analysis (CoA)
• HPLC Chromatogram
• Mass Spectrometry Confirmation
• Purity and Identity Verification Documentation
• Handling and Solubility Protocol
• Technical Research Support

Frequently Asked Questions — 5-Amino-1MQ EU

Can I Buy 5-Amino-1MQ in the EU and Europe?

Yes. We supply research-grade 5-Amino-1MQ with fast tracked international dispatch to all EU member states and wider European destinations including Germany, France, Netherlands, Spain, Italy, Poland, and beyond. All orders include full batch documentation and packaging designed to maintain compound integrity throughout transit. 5-Amino-1MQ is supplied strictly for laboratory research use only.

What is NNMT and Why is it a Significant Research Target?

Nicotinamide N-methyltransferase (NNMT) is an enzyme that methylates nicotinamide — a NAD+ precursor — using SAM as the methyl donor. Its metabolic significance lies in its position at the intersection of two critical regulatory systems: by consuming nicotinamide it reduces NAD+ precursor availability and the downstream activity of NAD+-dependent enzymes including sirtuins, and by consuming SAM it reduces cellular methylation potential and influences the epigenetic landscape. NNMT expression is elevated in obesity and in multiple cancer types — making it a research target of interest in metabolic dysfunction, epigenetic dysregulation, and cancer metabolism biology. 5-Amino-1MQ provides the primary small molecule tool for pharmacologically interrogating NNMT’s biological roles in these contexts.

How Does NNMT Inhibition Influence NAD+ Biology?

NAD+ is synthesised from nicotinamide through the salvage pathway — the primary NAD+ biosynthesis route in most mammalian tissues. NNMT competes with this pathway by methylating nicotinamide to 1-methylnicotinamide — diverting nicotinamide away from NAD+ synthesis. By inhibiting NNMT, 5-Amino-1MQ increases nicotinamide availability for the salvage pathway — raising NAD+ levels in cells and tissues where NNMT activity would otherwise reduce NAD+ precursor supply. This NAD+ elevation has downstream consequences for NAD+-dependent enzymes including SIRT1 and other sirtuins — connecting NNMT inhibition to the extensively researched NAD+/sirtuin metabolic regulatory axis.

What is the Relationship Between NNMT, SAM, and Epigenetic Regulation?

SAM (S-adenosylmethionine) is the universal methyl donor for cellular methylation reactions — including the histone methylation and DNA methylation reactions that constitute epigenetic regulation of gene expression. NNMT consumes SAM in the nicotinamide methylation reaction — reducing the SAM/SAH ratio and the methylation potential of the cell. In adipose tissue, this NNMT-driven reduction in methylation potential has been characterised as influencing histone methylation marks at genes regulating fat cell differentiation and metabolic function. 5-Amino-1MQ inhibition of NNMT restores methylation potential — with downstream consequences for the epigenetic landscape of treated cells that have been characterised in studies examining histone methylation changes and transcriptional programme shifts in adipose tissue models.

What is the Difference Between 5-Amino-1MQ and NMN in NAD+ Research?

5-Amino-1MQ and NMN (nicotinamide mononucleotide) both influence cellular NAD+ levels but through entirely distinct mechanisms. NMN is a direct NAD+ precursor that enters the salvage pathway to increase NAD+ synthesis — providing substrate-level elevation of NAD+. 5-Amino-1MQ inhibits NNMT to reduce nicotinamide diversion away from NAD+ synthesis — increasing NAD+ precursor availability indirectly. The two compounds are complementary rather than equivalent research tools — NMN for studying direct NAD+ precursor supplementation effects, and 5-Amino-1MQ for studying the consequences of NNMT-mediated nicotinamide competition with the salvage pathway. Combined use of the two compounds in research can help dissect the specific contribution of NNMT activity to NAD+ homeostasis in different tissue contexts.

Why is NNMT Elevated in Obesity and What Does This Mean for Research?

Studies have documented elevated NNMT expression in adipose tissue in obese versus lean states — with higher adipose NNMT activity associated with reduced NAD+ levels, impaired SIRT1 activity, and a gene expression profile favouring adipogenic and inflammatory programmes over oxidative metabolism. This obesity-associated NNMT upregulation has been proposed as a mechanism through which excess nutrient availability drives epigenetic and metabolic changes in adipose tissue that perpetuate metabolic dysfunction. 5-Amino-1MQ provides a pharmacological tool for studying whether NNMT inhibition can reverse these obesity-associated adipose tissue changes — and for characterising the precise mechanisms through which elevated NNMT activity contributes to metabolic dysfunction in dietary obesity models.

How is 5-Amino-1MQ Solubilised for Laboratory Use?

5-Amino-1MQ is a charged quinolinium compound with moderate aqueous solubility. It can be dissolved in sterile water or aqueous buffer for cell culture and in vivo research applications — a practical advantage over many small molecule research compounds requiring organic co-solvents. Prepare stock solutions in sterile water or PBS, filter sterilise if required for cell culture use, and store at -20°C protected from light. Refer to the included handling protocol for specific solubility and preparation guidance for your experimental system.

How Quickly is 5-Amino-1MQ Delivered to Europe?

Orders are dispatched promptly via tracked international courier. Delivery to EU and European destinations typically takes 3–7 working days depending on location, with packaging designed to maintain compound stability throughout transit.

Product Specifications

Parameter	Detail
Full Name	5-Amino-1-methylquinolinium
Type	Synthetic Small Molecule NNMT Inhibitor
Target	Nicotinamide N-methyltransferase (NNMT)
Inhibition Mechanism	Competitive — nicotinamide binding site
Primary Research Interest	NAD+ biology, adipose metabolism, epigenetics, obesity research
Purity	≥99%
Verification	HPLC & Mass Spectrometry
Form	Small Molecule Powder
Solubility	Aqueous — sterile water or PBS
Storage	-20°C, protected from light and moisture
Intended Use	Research use only

Research Disclaimer

5-Amino-1MQ is supplied exclusively for legitimate scientific research conducted within licensed laboratory environments. This product is not approved for human consumption, self-administration, or any therapeutic, clinical, or veterinary application. It must be handled solely by qualified researchers in compliance with applicable EU regulations, national legislation, and institutional ethics guidelines. By purchasing, you confirm this compound will be used exclusively for approved in vitro or pre-clinical research purposes.